Ampulloclitocybe clavipes

The medicinal mushroom Ampulloclitocybe clavipes
The club-footed clitocybe, Ampulloclitocybe clavipes (Pers.) Redhead.


Kingdom Fungi
Phylum Basidiomycota
Class Basidiomycetes
Order Agaricales
Family Tricholomataceae


Agaricus clavipes Pers.
Syn. meth. fung. (Göttingen) 2: 353 (1801)
Clavicybe clavipes (Pers.) Harmaja
Karstenia 42(2): 42 (2002)
Clitocybe clavipes (Pers.) P. Kumm.
Führ. Pilzk. (Zwickau): 124 (1871)

Common names

Club-footed clitocybe
Club foot
Clavate-stalked clitocybe
Clitocybe à pied en massue (French)
Hoteishimeji (Japanese)
Keulen-Trichterling (German)
Knotsvoettrechterzwam (Dutch)

My name is Austin Collins.

I've dedicated my life to Mushrooms.

I believe Mushrooms are the best kept secret when it comes to health and well being.

For that reason, I would like to share a company with you that in my opinion makes the best mushroom products on the market. 

The company is called Noomadic Herbals, my favorite supplement they make is called "Mushroom Total".

I take their products every day and they have helped me think better and have more energy. Give them a try.



Cap: 3-7 cm, applanate to slightly depressed, often slightly umbonate, grayish-brown to dark brown, lighter in color towards the margin, smooth to minutely subtomentose, somewhat greasy when moist, marginal zone somewhat decurved with an involute margin.
Gills: deeply decurrent, thin, crowded to subdistant, 3-6 mm broad, segmentiform, whitish, soon pale-cream to lemon-yellow, with entire, concolorous edge.
Stem: 3-6cm tall x 0.5-1.4 cm diameter, usually clavate towards base, but sometimes cylindrical to subclavate, stuffed, pale greyish cream, slightly to rather coarsely fibrillosestriate; context whitish, spongy in base of stem.
Smell: pleasant, sweet – similar to Iris flowers, even more so in dried specimens.
Taste: mild, indistinct.
Spore print: white.
Spores: sub-spherical to ellipsoid, smooth, hyaline, non-amyloid, with droplets, 6.0-9.5 x 4.0-5.0 µm.
Habitat: alone, in groups or gregariously under deciduous and coniferous trees (especially pine and spruce); common, widespread in the Northern hemisphere; summer-fall.
Edibility: some guide books say ‘edible with caution’. However, this mushroom is toxic in combination with alcohol due to presence of Type 6 toxins, similar to disulfram (Cochran and Cochran, 1978). [see also Coprinus atramentaria for a similar reaction caused by coprine] Furthermore, a toxicity text of this species showed that the crude extract was fatally toxic to albino mice 4 hours after consumption (Natour et al., 1993).

Description adapted from Noordelos et al., 1995, pg. 50.
Ampulloclitocybe clavipes Drawings
Drawings of the medicinal mushroom Ampulloclitocybe clavipes

Drawings of Ampulloclitocybe (formerly Agaricus) clavipes from Saunders et al., 1871, plate 31.

Fatty acid composition

GC and GC-MS analysis of the fatty acid methyl esters of Ampulloclitocybe clavipes revealed forty one fatty acids (FA). The mixtures comprise straight chained saturated FA with odd and even chain lengths in the range 10 to 26 carbon atoms, branched chained saturated FA with odd and even chain lengths from C13 to C17, straight chained monoenoic FA with odd and even chains ranging from C16 to C24, C16 and C18 straight chained dienoic FA and trienoic FA. Linoleic, oleic and palmitic acids accounted for 86.2% of the total FA content. In the dienoic fatty acid fraction, A. clavipes contains a series of dienoic isomers as minor components and traces: 11,14-18:2 (0.3%), 7,10-16:2, 7,10-18:2, 9,11-18:2 and 10,12-18:2 (0.1% each) and four positionally isomeric 18:2 FA with unknown positions of the double bonds (Marekov et al., 2007).

Bioactive compounds

Five fatty acid derivatives including three novel compounds were isolated from the clubfoot mushroom. These compounds were shown to inhibit aldehyde dehydrogenase in vitro (Kawagishi et al., 2002). This enzyme inhibition probably accounts for the coprine-like toxicity evident when this species is consumed with alcohol.
(E)-8-oxo-9-octadecenoic acid
(E)-8-oxo-9-octadecenoic acid (and the corresponding ethyl ester, not shown)
(E,E)-9-oxooctadeca-10,12-dienoic acid
(E,E)-9-oxooctadeca-10,12-dienoic acid
Novel AD inhibitor1
Novel C18 fatty acid derivative with α,β,γ,δ-dienone moiety
Novel C16 fatty acid ethyl ester

Clavilactones A-C (shown below) have been isolated from cultures of A. clavipes (Arnone et al., 1994).

Novel C16 fatty acid ethyl ester
Clavilactones A=1, B=4, C=5, benzoquinoid macrolidic fungal metabolites with antibacterial and antifungal activity.

Clavilactones D and E were later isolated from agar cultures (Cassinelli et al., 2000).

Clavilactone D
Clavilactone D
Clavilactone E
Clavilactone E

Clavilactone D potently inhibited epidermal growth factor receptor (EGFR) (IC50 = 5.5 µM) in a kinase assay (Merlini et al., 2000). The clavilactones represent a novel class of tyrosine kinase inhibitory activity and may hold promise as an anti-tumor therapy (Cassinelli et al., 2000).

In a large study of the specificity of fungal lectins with human and rabbit erythrocytes, A. clavipes showed unusual specificity against enzyme-treated erythrocytes (Pemberton, 1994).

Medicinal Properties

Antibacterial activity

Clavilactone B had antibacterial activity against Bacillus subtilis, B. cereus, Sarcina lutea (50 µg/disc); clavilactone A was only active against B. subtilis at 100 µg/disc (Arnone et al., 1994).

Antifungal activity

All clavilactones had antifungal activity, as determined using bioautography on Cladosporium cladosporioides and C. cucumerinum with amounts as low as 50 µg per plate (Arnone et al., 1994).

Antitumor activity

Polysaccharides extracted from the mycelial culture of A. clavipes and administered intraperitoneally into white mice at a dosage of 300 mg/kg inhibited the growth of Sarcoma 180 and Ehrlich solid cancers by 70% and 60%, respectively (Ohtsuka et al., 1973).


Mushroom Expert


Arnone A, Cardillo R, Meille SV, Nasini G, Tolazzi M.
Secondary mold metabolites. 47. Isolation and structure elucidation of clavilactones AC, new metabolites from the fungus Clitocybe clavipes.
J Chem Soc-Perk Trans 1. 1994 15: 2165-8.

Bousset M.
Sur la presence d’acide cyanhydrique chez Clitocybe clavipes et Rhodopaxillus nudus.
Bull Mens Soc Linn Lyon. 1941 10(10):154-5.

Cassinelli G, Lanzi C, Pensa T, Gambetta RA, Nasini G, Cuccuru G, Cassinis M, Pratesi G, Polizzi D, Tortoreto M, Zunino F.
Clavilactones, a novel class of tyrosine kinase inhibitors of fungal origin.
Biochem Pharmacol. 2000 59(12):1539-47.

Cochran KW, Cochran MW.
Clitocybe clavipes – Antabuse-like reaction to alcohol.
Mycologia. 1978 70(5):1124-5.

Jarvis MC, Miller AM, Sheahan J, Ploetz K, Ploetz J, Watson RR, Palma Ruiz M, Pascario V, Carlos A, Garcia Alvarado J, Lopez Ramirez A, Orr B.
Edible wild mushrooms of the Cofre de Perote region, Veracruz, Mexico: An ethnomycological study of common names and uses.
Economic Botany. 2004 58(Suppl. S):S111-S115.

Kawagishi H, Miyazawa T, Kume H, Arimoto Y, Inakuma T.
Aldehyde dehydrogenase inhibitors from the mushroom Clitocybe clavipes.
J Nat Prod. 2002 65(11):1712-4.

Marekov I, Antonova D, Gyosheva M, Momchilova S, Nikolova-Damyanova B.
Fatty acid composition of lipids from two macrofungal species belonging to genus Clitocybe.
CR Acad Bulg Sci. 2007 60(7):757-762.

Merlini L, Nasini G, Scaglioni L, Cassinelli G, Lanzi C.
Structure elucidation of clavilactone D: an inhibitor of protein tyrosine kinases.
Phytochem. 2000 53(8):1039-41.

Natour RM, Salhab AS, El-Moumani AR, Saba EF.
Wild mushroom in Jordan.
Dirasat Series B Pure and Applied Sciences. 1993 19(2):47-60.

Noordeloos ME, Boekhout T, Vellinga EC, Arnolds EJM. 1995.
Flora agaricina Neerlandica; critical monographs on families of agarics and boleti occurring in the Netherlands, v.3.
CRC Press
Google Books

Ohtsuka S, Ueno S, Yoshikumi C, Hirose F, Ohmura Y, Wada T, Fujii T, Takahashi E.
Polysaccharides having an anticarcinogenic effect and a method of producing them from species of Basidiomycetes.
UK Patent 1331513, 26 September 1973.

Pemberton RT.
Agglutinins (lectins) from some British higher fungi.
Mycol Res. 1994 98(3):277-90.

Redhead SA, Lutzoni F, Moncalvo JM, Vilgalys R.
Phylogeny of Agarics: partial systematics solutions for core omphalinoid genera in the Agaricales (Euagarics).
Mycotaxon 2002 83:19-57.

Saunders WW, Smith WG, Bennett AW. 1871.
Mycological Illustrations: Being Figures and Descriptions of New and Rare Hymenomycetous Fungi.
London: John van Voorst.
Digitized in Google Books

Yamaura Y, Fukuhara M, Kawamata S, Satsumabayashi H, Takabatake E, Hashimoto T.
Effects of Clitocybe clavipes extract on the components and enzymes related to ethanol metabolism in mice.
J Food Hyg Soc Jpn. 1986 27(5):522-7.

Yamaura Y.
Classification of poisonous mushrooms according to their biochemical effects in mice.
Nippon Eiseigaku Zasshi-Japanese Journal of Hygiene. 1988 43(2):669-78.

Leave a Comment